Journal: Journal of Functional Biomaterials

Article Title: FeMn with Phases of a Degradable Ag Alloy for Residue-Free and Adapted Bioresorbability

doi: 10.3390/jfb13040185

Figure Lengend Snippet: Effect of degradation supernatant on the growth of the reference strain Escherichia coli (ATCC ® 25922). Results represent effects caused by the previous degradation in 3 mL Mueller–Hinton broth. Mean value +/− standard deviation from four replicates. The optical density of the untreated control was set as 100%. Repeated measures analysis of variance (ANOVA) summary with Holm–Šídák’s multiple comparisons test in comparison to control; p < 0.001 = * (GraphPad Prism 9.1.0.; GraphPad Software, LLC, San Diego, CL, USA).

Article Snippet: Standard strains of Escherichia coli ( E. coli ; ATCC ® 25922, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) are used since these bacteria are common etiologic agents of clinical infections involved in bone tissue infections related to implants.

Techniques: Standard Deviation, Control, Comparison, Software

Journal:

Article Title: Isolation and Characterization of Bacteria Capable of Tolerating the Extreme Conditions of Clean Room Environments ▿

doi: 10.1128/AEM.03007-06

Figure Lengend Snippet: Intracellular ATP content of several microbes that were isolated from spacecraft or other clean environmental surfaces

Article Snippet: No signal above background was obtained. table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Organism Strain a Intracellular ATP content (10 −18 mol/CFU) RLU/CFU or RLU/spore Avg RLU/CFU Gram-negative bacteria 1.34 Acinetobacter radioresistens MO-50v1 1.91 0.98 Escherichia coli ATCC 25922 2.39 1.23 Methylobacterium radiotolerans MSFC 2M5-R1 3.70 1.90 Proteus mirabilis ATCC 29906 2.13 1.10 Pseudomonas aeruginosa ATCC 27853 1.84 0.95 Pseudomonas stutzeri JPL-1 1.44 0.74 Pseudomonas graminis JPL-8 5.45 2.80 Sphingomonas yanoikuyae MSFC 4M9-R1 1.98 1.02 Gram-positive bacteria 4.49 Bacillus subtilis ATCC 9372 8.45 4.34 Bacillus macroides JPL-4 10.27 5.28 Kokuria rhizophila JPL-9 11.99 6.16 Micrococcus luteus IFO 3333 8.28 4.26 Staphylococcus aureus ATCC 25923 5.77 2.97 Staphylococcus capitis JPL-2 8.85 4.55 Staphylococcus epidermidis JPL-7 7.47 3.84 Yeasts 263.50 Candida utilis NISL 3727 677.00 348.07 Saccharomyces cerevisiae NISL 3398 348.00 178.92 Spores 0.0016 Bacillus circulans ATCC 4513 0.0032 0.0016 Bacillus subtilis ATCC 6633 0.0037 0.0019 Lactobacillus buchneri ATCC 11577 0.0022 0.0011 Bacillus subtilis vegetative cells Autoclaved ATCC 6633 No RLU b NG c UV 254 -killed ATCC 6633 No RLU NG H 2 O 2 -sterilized ATCC 6633 No RLU NG Open in a separate window a Abbreviations used in strain designations: MO, Mars Odyssey spacecraft; ATCC, American Type Culture Collection; MSFC, Marshall Space Flight Center; JPL, Jet Propulsion Laboratory; IFO, Institute of Fermentation-Osaka, Japan; NISL, Noda Institute for Scientific Research, Noda, Japan. b When measured for bioluminescence after removing extracellular ATP, no luminescence was recorded. c NG, no growth when grown on TSA or R2A agar.

Techniques: Isolation, Bacteria

Journal: bioRxiv

Article Title: Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

doi: 10.1101/2020.08.11.246652

Figure Lengend Snippet: a , Schematic of coupling the peptidomimetic antibiotic Thanatin to the singlet oxygen generator (SOG) thiorhodamine. b , Volcano plot showing relative abundance of LUX-MS quantified proteins from Thanatin-SOG treated Escherichia coli illuminated for 15 min with and without Thanatin competition. Blue dots represent significantly enriched proteins and red dots represent the direct binding targets of Thanatin previously found by photo-crosslinking experiments. The main targets and selected surfaceome interactors are highlighted. c , schematic representation outlining the molecular mechanism of action of Thanatin in E.coli cells with spatial context. A list of MS-quantified proteins ( Supplementary Table 3 ) and a fully interactive volcano plot ( Supplementary Data 4 ) are provided to facilitate data exploration.

Article Snippet: Escherichia coli cells (Migula) Castellani and Chalmers (ATCC: 25922) were incubated with 3 μM Thanatin-SOG in the presence or absence of 30 μM unconjugated Thanatin derivative for 30 min at 37 °C in the dark to minimize background light-induced oxidation.

Techniques: Binding Assay

Journal: ACS Infectious Diseases

Article Title: Insights into the Spectrum of Activity and Mechanism of Action of MGB-BP-3

doi: 10.1021/acsinfecdis.2c00445

Figure Lengend Snippet: Thermal melt curves of gDNA (salmon) and gDNA:MGB-BP-3 Complex. (A) Exemplar melt curve from one experimental repeat, visually representing the different melt curves of gDNA and the gDNA:MGB-BP-3 Complex. Data has been fitted with a Boltzmann distribution. (B) Melting temperatures of gDNA and gDNA:MGB-BP-3 Complex calculated from fitted Boltzmann distributions using OriginPro 2021. All values are an average for n = 4 experimental repeats with an error of ±1 °C.

Article Snippet: Bacterial genomic DNA (ATCC 43300, DSM 13661; ATCC 27853, DSM1117; ATCC 700603, DSM 26371; ATCC 25922, DSM 1103; ATCC 19606, DSM 30007; ATCC 51299, DSM 12956; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) or salmon genomic DNA (deoxyribonucleic acid sodium salt from salmon testes, D1626, Merck) dissolved in 1 mM phosphate buffer pH 7.4 (containing 0.27 mM potassium chloride, 13.7 mM sodium chloride) to a concentration of 100 μg/mL in SybrSafe (SYBR Safe DNA Gel Stain, ×10,000 in DMSO, S33102 Invitrogen) was used as supplied by the manufacturer in DMSO, and MGB-BP-3 was prepared as 10 mM stock in DMSO.

Techniques:

Journal: ACS Infectious Diseases

Article Title: Insights into the Spectrum of Activity and Mechanism of Action of MGB-BP-3

doi: 10.1021/acsinfecdis.2c00445

Figure Lengend Snippet: Remaining Fluorescence (%) of SybrSafe Probe upon Addition of MGB-BP-3 to gDNA a

Article Snippet: Bacterial genomic DNA (ATCC 43300, DSM 13661; ATCC 27853, DSM1117; ATCC 700603, DSM 26371; ATCC 25922, DSM 1103; ATCC 19606, DSM 30007; ATCC 51299, DSM 12956; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) or salmon genomic DNA (deoxyribonucleic acid sodium salt from salmon testes, D1626, Merck) dissolved in 1 mM phosphate buffer pH 7.4 (containing 0.27 mM potassium chloride, 13.7 mM sodium chloride) to a concentration of 100 μg/mL in SybrSafe (SYBR Safe DNA Gel Stain, ×10,000 in DMSO, S33102 Invitrogen) was used as supplied by the manufacturer in DMSO, and MGB-BP-3 was prepared as 10 mM stock in DMSO.

Techniques: Fluorescence

Journal: Food Technology and Biotechnology

Article Title: Autochthonous Human Milk Lactobacillus Strains as Potential Probiotic Starter Cultures

doi: 10.17113/ftb.64.01.26.9074

Figure Lengend Snippet: Antimicrobial activity of lactic acid bacteria (LAB) strains against: a) test microorganisms, and b) related LAB strains tested by the turbidimetric method. Different letters above bars indicate statistically significant difference (p<0.05) between the LAB strains and the same test microorganism, i.e. a related LAB strain

Article Snippet: The antimicrobial activity of the selected LAB isolates from human milk was tested against test microorganisms ( Staphylococcus aureus ATCC ® 25925 TM , Listeria monocytogenes ATCC ® 19111 TM , Escherichia coli ATCC ® 25922 TM and Salmonella Typhimurium FP1) and related LAB strains ( Lactobacillus helveticus M92, L. lactis ssp. lactis LMG 9450 and Enterococcus faecium ATCC ® 9430 TM ) cultured in the appropriate medium until their A 620 nm reached 0.5 and 1.0, respectively.

Techniques: Activity Assay, Bacteria

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Disc diffusion assay of cell-free culture supernatants (CFCSs), obtained from W. coagulans ATCC 7050, W. coagulans MA42, W. coagulans S5, and W. coagulans P13 cultured in mMRS broth at 37 °C under static conditions for 24 h, against E. coli ATCC 25922, S. Typhimurium TISTR 292, B. cereus TISTR 747, and S. aureus TISTR 746. Control (M): uninoculated mMRS broth. Un-neutralized CFCS ( A ) and neutralized CFCS (pH 7) ( B ).

Article Snippet: W. coagulans MA42 was the most effective in inhibiting target pathogens, with 17.50 ± 1.50, 16.00 ± 1.00, 14.50 ± 0.50, and 8.50 ± 0.50 mm clear zones against E. coli ATCC 25922, S. Typhimurium TISTR 292, B. cereus TISTR 747, and S. aureus TISTR 746, respectively, showing an inhibition zone better than W. coagulans ATCC 7050, as shown in A.

Techniques: Diffusion-based Assay, Cell Culture, Control